Cell Proliferation Analysis

Guava Technologies has introduced a new simple non-radioactive assay designed to improve the performance and sensitivity of cell proliferation assays compared to traditional population-based methods such as 3-Thymidine and MTT (or XTT). The Guava CellGrowth assay, when used in conjunction with the Guava EasyCyte System, provides cell proliferation analysis at the single-cell level, offering cell proliferation assessments without the use of more complex fluorescence methods out to five generations. The Guava CellGrowth Assay uses a well-characterized cell tracking dye that diffuses freely into cells and is retained within the cell without affecting cellular function. The dye is not transferred to adjacent cells. The assay uses two dyes, a cell permanent painting dye and a cell impermanent DNA binding dye, to distinguish live or dead proliferated cells from live or dead resting cells. For each round of cell division, the relative fluorescence intensity of the dye is decreased by half. The assay is ideal for monitoring the growth of primary cultures.