05/25/2005 • Analytics • Laboratory appliances

Rapid, Accurate Determination of Baculovirus Titre

Guava Technologies presents a new technical paper describing a novel flow cytometric type assay that provides rapid, accurate determination of Baculovirus titres. Recombinant protein expression using the Baculovirus vector system is a powerful tool for the production of therapeutics, diagnostics and reagents. To maximise efficiency of protein production, and thereby reduce costs, optimal manufacturing parameters are typically determined using the Baculovirus titre assay. The technical paper discusses the benefits and shortfalls of traditional Baculovirus titre methods including real-time PCR, end point dilution and plaque assays. Results obtainable by these methods are compared to the flow cytometric type assay carried out using a Guava PCA-96 system. This system is an affordable, ultra-compact system ideally suited for automatic, 96-well microplate and multiple microcentrifuge tube analysis of cell lines and compounds. Intuitive in operation, the system provides precise, accurate and easy-to-read results with often less than one day of training.
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Guava Technologies inc.

25801 Industrial Blvd.
CA 94545 Hayward

Phone: 001/510/5761408
Fax: 001/510/5761500