Integrated Solutions for ADME Screening

The SpectraMax M5 allows researchers to increase productivity using multiple detection modes in one powerful, compact instrument. Utilizing the top-of-line optical performance of the SpectraMax product line, the platform is suitable for various applications, such as fluorescence (FI), absorbance (Abs), luminescence (Lum), Time-Resolved Fluorescence (TRF) and Fluorescence Polarization (FP) assays. With its broad assay capability, this system can be used as a research tool to optimize assays in early ADME or quality control, or used as a screening instrument. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. The system also gives users the flexibility to choose wavelengths between 200 nm and 1,000 nm. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and well-area scanning. For life sciences research, the system is well suited for ELISA, nucleic acid, protein quantitation, enzymatic, cell viability and proliferation assays. Kinase, reporter gene, FRET and other homogeneous and heterogeneous assays can also be run. We have also launched two new reagent products for ADME assay screening – Transil Membrane Affinity and Transil Human Serum Albumin (HSA) Binding assay kits. The Transil assay kits provide lead optimization and high-throughput screening laboratories with fast, automatable, direct, biologically relevant solutions to increase the throughput of ADME-related compound profiling. The Transil Membrane Affinity assay provides data that correlates better to in vivo studies compared to the more conventional water/octanol partitioning assays because it uses beads which are coated with a lipid bilayer that mimic interactions between compounds and cell membranes. The results are equivalent to the liposome “gold standard” method, without the tedious preparation of liposomes. The Transil HSA Binding assay kit makes routine, high-throughput HSA binding characterization easy because it contains beads which have been coated with human serum albumin and pre-dispensed into a microplate format. The pre-dispensed format increases throughput via parallel sample preparation and assay reading on Molecular Devices’ SpectraMax microplate readers. With the Transil assays, there is virtually no methods development required, as each kit contains data analysis routines for processing high-throughput screening of drug candidates for early ADME characterization. Furthermore, the assays share nearly identical sample workup steps, so steps developed for one assay are im mediately applicable to the other. The Transil assay kits enable screening of compound libraries for membrane affinity and HSA binding early ADME, properties which are not possible with current low-throughput methods. These new assays require only one sample concentration, which simplifies automation. Additionally, turnkey data analysis offers researchers compound data within hours of synthesis or secondary screening. Because the company has integrated these assays with its SpectraMax microplate reader line, a more complete solution can be offered. For more information on this complete solution for ADME screening, visit www.moleculardevices.com or visit our booth in Hall 2 at Biotechnica, October 18–20, 2005, Hanover, Germany.