Separating Multifluorescent Labels

With Release 3.2 Add On for its confocal microscope systems, Carl Zeiss offers new functions for separating multifluorescent labels in biological specimens. MultiChannel Unmixing now permits the separation of spectrally overlapping fluorescent signals with the LSM 510, LSM 510 NLO and the LSM 5 Pascal. This function of the LSM software is based on the simultaneous detection of up to three different fluorescent signals using different detectors. The actual distribution of fluorescent signals in the specimen is then calculated by comparing the relative signal intensities for each image pixel. This enables these laser scanning microscopes to visualize even complex combinations of fluorescence dyes. Furthermore, the new software extends the possibilities of detecting spectral signatures of fluorescent markers in multi-photon microscopy.