Quantitative FRET Measurements
AxioVision FRET is the innovative solution for FRET (Fluorescence Resonance Energy Transfer) - and the first complete system specifically developed for this new technique. It includes complete functionalities for all steps, from image acquisition and microscope control to measurement, display and documentation. AxioVision FRET offers easy, practice-driven operation combined with high flexibility - from region definition by thresholds, circles or
freehand up to the individual user interface configuration.
FRET Determination Using Correction or Bleaching Methods
By combining optical microscopy with FRET, it is possible to obtain quantitative temporal and spatial information about the binding and interaction of proteins, lipids, enzymes, DNA, and RNA in vivo. These interactions usually are below the normal resolution of a light microscope and therefore of special interest in cell and developmental biology. AxioVision FRET provides the currently most important applied techniques for the correction and measurement of the FRET amount in a single software tool: Youvan's, Gordon's, Xia's and acceptor ratio method for intensity or ratio images, acceptor bleaching for FRET percentages.The Steps:
- Acquiring reference images
- Measuring the background
- Selecting areas of interest (ROI)
- Acquiring FRET raw data
- Measuring the FRET amount
- FRET-based color coding
- Graphic display in histogram
- Storing data in tables
This product information
is expired!
Use our search-function for current products ...
is expired!
Use our search-function for current products ...
