Four Color Imaging

Jackson Immuno Research Laboratories has recently added Dylight 405 to its range of conjugated secondary antibodies. Dylight 405-conjugated antibodies are excited maximally at about 400 nm and fluoresce with a peak at about 421 nm. They are very bright and photo stable, but their use is limited to confocal microscopes equipped with a 405 nm laser and appropriate emission filter.

It is now possible to perform effective four color imaging with excellent color separation, good photo stability and high sensitivity in both aqueous and permanent mounting media. The combination of Dylight 405, Dylight 488, Rhodamine Red-X and Dylight 649 maximizes color separation.