Removal of Hydrophobic Contaminants

Sartorius Stedim Biotech is launching a membrane adsorber for hydrophobic interaction chromatography (HIC). Sartobind Phenyl membrane adsorbers allow increased flow rates. This ensures faster processes, and can be used for process upgrades in purification and polishing of therapeutic proteins. It provides the advantages of an HIC ligand adsorber, while showing the specificity and behavior of a conventional HIC matrix. The hydrophobic phenyl ligand is covalently attached to the >3 µm cellulose membrane. With a binding capacity of 0.4 mg/cm² (15 mg/ml), the membrane adsorber can be used for antibody medium scale capture. Its key application is removal of hydrophobic aggregates that pose the risk of unwanted immune system response when patients are treated with therapeutic proteins. Detergent removal and large molecule/bioparticle purification are further application options for HIC membranes.