Orthogonal Liquid-Liquid Chromatography

In these days of acetonitrile shortages and that which is available being priced so highly, perhaps the time has come to consider an alternative preparative liquid chromatography technique. High performance CCC is a high resolution combined with high processing capacity technique that any chromatographer can readily adopt is now commonly being used on a wide range of compounds small and large synthetic molecules, peptides and natural products. The reason for this makes even more sense in these times of acetonitrile scarcity; the technique uses only 1/10th the solvent per gram injected that preparative scale HPLC uses and it uses freely available solvents such as hexane, ethyl acetate, methanol or water in its operation. Fractions are produced at high concentration further reducing the required work-up time post the purification.