07/02/2009 • Microscopy / Imaging

Advanced Objective for Multiphoton Microscopy

Olympus has developed a new 25x water immersion objective specifically designed for multiphoton imaging. It offers researchers outstanding deep-imaging and high resolution performance with the Olympus FluoView FV1000MPE multiphoton laser-scanning microscope system.

  • Superior Optics: The new Olympus XLPlan N 25x objective has been designed with a high numerical aperture (NA) of 1.05 for high-resolution multiphoton microscopy. It can be used for tissue samples with cover slips and for investigations, such as patch clamping, where the sample cannot be covered. As a result, higher z-resolution and significantly increased brightness is achieved for all deep imaging applications, compared to similar objectives already on the market.
  • Superior Functionality: With a working distance of 2 mm and a large 35-degree access angle available for patch clamping, this objective is excellent for neurophysiology and related applications. In addition, a super-wide field of view (27.5) aids researchers in obtaining more information from each multiphoton experiment. Furthermore, a correction collar compensates for refractive index mismatches, allowing excellent axial and spatial resolution hundreds of microns into living brain samples.
  • Superior Multiphoton Microscopy: This is the first time ever that a life science objective has been dedicated to the fast growing multiphoton imaging area. As a result it has been designed to maximise the effectiveness of the multi-photon process, with chromatic aberrations corrected within Ti:Sa IR-laser wavelength range 680-1100 nm. In addition, new optical coatings allow the objective to provide outstanding performance in the IR range, with transmittance of more than 82% from 400 to 1000 nm. This results in at least 5x the normal brightness levels during multiphoton excitation, combined with more efficient collection of emitted light, providing greatly enhanced instrument sensitivity and higher signal-to-noise ratios.
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